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This step and the next step of Western blotting were necessary, because the proteins evaluated in the Kinex TM KAM 1.0 antibody microarrays were not denatured, which increases the opportunity for false positives and false negatives due to antibody cross-reactivity, protein-protein interactions, and blocked epitopes in protein complexes

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It can serve as a coping mechanism to manage stress and anxiety that may arise during this period