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Upon reaching 90% confluency, cells were rinsed with Dulbeccos phosphate-buffered saline (DPBS) without calcium and magnesium then detached using 1.5 mL of 0.25% trypsin EDTA/DPBS with polyvinylpyrrolidone for 1.5 min at 37 C

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Abbreviations Apoe: Apolipoprotein E E-cigarette: Electronic cigarette FDA: Food & Drug Administration HPHC: Harmful or potentially harmful chemical NNAL: 4-Methylnitrosamino)-1-(3-pyridyl)-1 butanol NNN: N 1 -Nitrosonornicotine Nrf2: Nuclear factor erythroid factor 2 RRP: Reduced risk product TEER: Trans-epithelial electric resistance References WHO

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Flora of North America - Card #5 Inside the cabin on the south-most windowsill