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A total of 1.2 g of RNA was utilized for reverse transcription, accomplished using the MMLV Reverse Transcriptase kit (M1701, Promega, Walldorf, Germany), Random Hexamer Primers (48,190,011, Thermo Fisher), and RiboLock RNase Inhibitor (EO0384, Thermo Fisher)

Smokers may also notice wounds heal more slowly

The myosin II isoform 10 level was increased to about 1.2-fold after exposure to nicotine [33]

The study had also found that a large proportion of students (43.7%) had previously tried e-cigarettes 18 , highlighting that many young adults have previously experimented with e-cigarettes

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