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The plant was first brought to Europe by Christopher Columbus in the late 15th century and quickly became popular, with smoking and chewing tobacco spreading throughout the continent

"It has been a very long road, but at the same time, that's the time it took to make the evidence in the trial and present all we had to say to the judge," said Mario Bujold, executive-director of the Quebec Council on Tobacco and Health

In particular, the number of younger smokers, aged between 12 and 17, and between 18 and 25, has declined significantly over the past 20 years

Additional files Additional file 1: (download DOCX The typical symptoms and polymerase chain reaction detection of five begomoviruses and betasatellite in tobacco samples