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Protein determination by sandwich enzyme-linked immunosorbent assay IL-6 was quantified by ELISA assay (Biotech Co., Ltd) in the supernatants of control and vitiligo melanocytes and fibroblasts according to the manufacturers protocol

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The authors thank the Center for Advanced Equipment and Educational Support, Faculty of Agriculture, Kyushu University for the use of the ECL-detection imaging system and the plate-reader

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[DOI] [PMC free article] [PubMed] [Google Scholar] Moon DO, Kim MO, Choi YH, Hyun JW, Chang WY, Kim GY