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Thirty five milliliters (mL) of blood was drawn once from both healthy volunteers and individuals with HIV infection

Wu et al., 2010

Timing of Dose: It is recommended to administer PT-141 at least 30 to 45 minutes prior to anticipated sexual activity.In female trials, onset of effect was noted around 30-60 minutes after injection, and similarly men report that it takes about half an hour (sometimes up to an hour) to start feeling the effects

Nature 312 , 724729 (1984)