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Higher-order structure characterisation through techniques such as NMR, circular dichroism (CD), and FTIR Stability studies to assess degradation pathways including oxidation, deamidation, and oligomerisation Importantly, formulation components can interfere with detection (e.g., UV-active excipients), requiring careful method design and sample preparation strategies such as buffer exchange

Cancer cells may metabolize glutamate to -ketoglutarate through one of two different pathways (glutamate dehydrogenase or aminotransferases), with aminotransferases potentially supporting a more biosynthetic and pro-growth phenotype

IDH1/2 mutations sensitize acute myeloid leukemia to PARP inhibition and this is reversed by IDH1/2-mutant inhibitors