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Nle 1 -AngIV (Nle-Tyr-Ile-His-Pro-Phe), d-Nle-YI, acetyl-NleYIH, -amino butyric acid-YIH, NleYI-amide, and N-hexanoic-Tyr-Ile-(6)amino-hexanoic amide (dihexa) were synthesized by 9-fluorenylmethoxycarbonyl-based solid-phase methods in the Harding laboratory

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Furthermore, alignment analysis also demonstrated that Asn (N), Gly (G), Arg (R), Pro (P), Thr (T), Tyr (Y), Lys (K), Ala-Ser (AS), Cys-Gly (CG), Phe-Pro (FP), Glu-Pro (EP), Leu-Lys (LK), Lys-Phe (KF), Asn-Gly (NG), Asn-Gln-Phe (NQF), and Trp-Asn-Phe (WNF) residues were strictly conserved in all aligned sequences, indicating their potential functions in enzyme activity and/or stability (Supplementary Figure S3)

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