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Some researchers prefer injecting in the morning to align the metabolic effects with daytime activity

Brains were removed, postfixed overnight, and allowed to sink in 2.5% paraformaldehyde supplemented with 30% sucrose in PBS solution

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It is also part of good clinical practice because metabolic, endocrine, and inflammatory markers can shift during meaningful weight change, especially in the first few months

Therapeutic inertia in the treatment of hyperglycaemia in patients with type 2 diabetes: A systematic review