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Spots were dried and plates were incubated for 48 h at 32 C

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Additionally, genetic variants in the phosphodiesterase 8B ( PDE8B ) gene, which encodes a high-affinity cAMP-specific phosphodiesterase, as genetic modulators of TSH levels (30, 31), and in the TSH receptor (32) may also influence the setpoint of the HPT axis, potentially contributing to diminished cellular responsiveness to circulating thyroid hormones

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Then I went into another joke very gingerly, just going this, that, and they laughed at that