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Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

If its the next day, however, youre better off making a new buffer with fresh reduced glutathione instead of using your old one

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Severe abdominal pain , particularly if it is persistent, worsening, or accompanied by fever, vomiting, or inability to keep fluids down, requires urgent assessment