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Peptide cleavage and purification Dried peptide-containing resin was suspended in 1.5 ml cleavage cocktail (2.5% DODT, 2.5% H 2 O, 2.5% TIPS in TFA) per 100 mg resin and agitated for 2 h
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This cell line expresses a Ca 2+ sensitive photoprotein and a cAMP gated channel
Similar to liraglutide, semaglutide (Novo Nordisk) is a fatty-acylated GLP-1 analogue with a stearic-diacid at lysine position 20, linked via a di-aminoethoxy, -glutamic acid spacer, and an aminoisobutyric residue at position 2 to provide protection from DPP-IV degradation [28]
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