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A subsequent study found that circulating ghrelin levels were elevated in children and adult subjects with PWS compared to both lean and obese controls as a result of increased active AG, whereas UAG was relatively low

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Blocking was performed using 2% BSA in PBS for 10 min at room temperature, and slides were incubated with streptavidin CY3 (ThermoFisher Scientific #438315) for 45 min at room temperature in a humid chamber