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The substrate solution is added to each well

Aliquoting into smaller vials with fewer punctures per vial is the best strategy for protocols requiring many doses

There is a further point that the combination-first development strategy makes stubbornly hard to resolve

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In the studies on acclimatization of walnut trees, it was found that lowering relative humidity (application of KCl for 2 and 4 weeks before transferring the micropropagated plantlets to ex vitro conditions) in the growth vessels effectively increased the efficiency of ex vitro acclimatization (Asayesh et al