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Significant changes in TCA intermediates fumarate and succinate, and in the secondary TCA metabolites succinyl-carnitine and methyl succinyl-carnitine were observed, arguing that the potential anti-inflammatory properties of these metabolites are mediated by metabolic rewiring

Ethics approval and consent to participate All human breast tumor samples were obtained from patients with informed consent

After lysis and centrifugation, the proteins were isolated by affinity chromatography with Ni 2+ -NTA agarose resin and the 6xHis tag was cleaved using TEV protease except for UBA1

However, when sourcing a research compound, one can acquire either the peptide alone (GHK) or the pre-complexed form (GHK-Cu)