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The distal end (S49 ECD ) of the helix was more mobile than the proximal one (E34 ECD ), which had an overall tendency to remain in the proximity of the TT-OAD2-binding site, driven by transient interactions with the ligand (Extended Data Table 1) and hydrogen bonds with the R299 ECL2 side chain (Extended Data Table 2)

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Therefore, it confirmed the emergence of the subpopulation of swollen cells with the lower diameter and higher thickness