GST Pull-Down assays For the GST pull-down assay, dilutions of the sonicated whole cell extract from GST only control, GST-Taf4, GST-Ada1, GST-HFDTaf4, GST-HFDAda1, Taf12LFLAG 3 and Taf12-FLAG 3 bearing strains were run on an SDS-PAGE gel along with marker dilutions to estimate the amount of fusion protein that was expressed in the lysate
That means the study met its main goal, but those watching the weight-loss drug race closely had pegged their hopes on an average result for CagriSema of at least 25% weight loss, which would set a new bar for a field thats already been revolutionized by GLP-1 drugs
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