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The film was further dried under a constant flow of Nitrogen 3.5 (Messer Poland Ltd., Chorzow, Poland) for 3 min

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Another uncertainty is that the underlying science is still in flux and debate rages among academic researchers over many details of how caloric restriction works

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High doses of IV fluids can also lead to more serious problems, such as kidney damage, irregular heart rhythms, blood pressure fluctuations, gastrointestinal discomfort, and nerve damage