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The quantified RNA was used to construct the cDNA library and sequenced on an Illumina Novaseq 6000 platform (Illumina, San Diego, CA, United States) at LC-Bio Technologies Co., Ltd., (Hangzhou, China)

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For semaglutide (7-day half-life), that is 28-35 days

For amplifying the immunoreactions, the sections were incubated for 40 min with biotinylated secondary antibody immunoglobulins, diluted at 1:200, followed by a preformed Avidin and Biotinylated horseradish peroxidase macromolecular complex (ABC) (Code nr