UV exposure: Melanotan II increases melanin without UV, but some users combine with minimal UV exposure
5 days/week (~12 mg/day): ~13 vials 3/week (2 mg): ~12 vials 16 weeks: ~3 vials Insulin Syringes (U-100, 1 mL) Per injection: 1 syringe 8 weeks (5 days/week): ~40 syringes 16 weeks (5 days/week): ~80 syringes Bacteriostatic Water (10 mL bottles) Use ~3.0 mL per 100 mg vial for reconstitution
not knockdown in clock neurons lengthened locomotor periods, reduced rhythm robustness, and decreased expression of several clock genes, including tim , per , and Clock (Mahesh et al., 2020
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Then cells were diluted with 0.51 ml assay buffer, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)
