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ABHD11 purification To purify ABHD11-Flag protein, 1 10 8 HeLa cells expressing ABHD11-Flag were lysed in 1 ml lysis buffer (50 mM TrisHCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 Roche complete protease inhibitor cocktail) and rotated at 4 C for 1 h

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Effects of Methylcobalamin Injection typically wear off between 48 to 72 hours, though this varies from person to person

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