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What was studied: Cardiovascular death and worsening of heart failure over a median follow-up of roughly two years Outcome: Tirzepatide reduced the combined risk of cardiovascular death and worsening heart failure events by approximately 38%, driven largely by a 46% reduction in worsening heart failure events requiring hospitalization or urgent care
WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA
P., & Scheett, T
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