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Once niacinamide is applied, its broken down into the form of vitamin that our cells can use: the coenzyme nicotinamide adenine dinucleotide

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Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

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