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Iterative cycles of real-space refinement and analysis were performed in Phenix 49 and CCP-EM modules 50,51 were used throughout the structure-building process for map sharpening/blurring and for structure analysis

By kinetic analysis of single, double, and triple mutants, as well as from studies of reaction products, it was established that the enzyme A216H catalyzes the hydrolysis of the thiol-ester substrate by a mechanism that includes an acyl intermediate at the side chain of Y9

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200 mg) produce evidence for beta-1 stimulation in patients (rxlist.com) This mechanism is associated with cardiovascular adverse effects, including clinically significant increases in blood pressure, tachycardia, and hypertension (Segovia & Crisp 2021)