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A metagenome-wide association study of gut microbiota in hepatitis B virus-related cirrhosis in northwest China

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The cells were cultured in RPMI-1640, DMEM, MEM F-12K, or McCoys 5 A medium (Meilunbio, China) supplemented with 10% FBS (Gibco, USA) and 1% antibiotics (Gibco, USA)

In the presence of peroxidase, the Amplex Red reagent reacts with H 2 O 2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin, which can be detected at a wavelength of 560 nm spectrophotometrically

The overactive immune response then causes more cellular damage, exacerbating the problems from the mycotoxins