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Cells were then washed 3 times with PBS, then washed with DIPA staining solution for 5 min, followed by glycerol blocking, and then observed using a fluorescence microscope ( 2.7 Statistical analysis Statistical analysis and graph production were performed using GraphPad Prism (GraphPad Software) version 6, and Microsoft Excel was used to perform ANOVA for data analysis

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With 0.5 mL, concentration is 10 mg/mL, and a 0.25 mg dose is 2.5 units

Circulation 145 , 17381740 (2022)

[13] If less than 2 days until the next scheduled dose, skip the missed dose and take the next dose on the regular day