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Spatial transcriptomics combined with single-cell RNA sequencing identified high expression levels of ferroptosis-related genes, including TFRC, NCO4, and SLC3A2, in granulosa cells of patients with ovarian senescence, while GPX4 expression was notably low ( BNC1 mutations that lead to premature follicular activation and atresia (Zhang et al., 2018)

Clark et al., 2016)

Step 1 Prepare all reagents, samples, and working standards as directed in the previous sections

Protein lysates from cellular or tissue samples were prepared using cold RIPA lysis buffer

Impact of Semaglutide on Body Composition in Adults With Overweight or Obesity: Exploratory Analysis of the STEP 1 Study