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Samples were prepared with TA (30, 150, and 300 g/mL) [26], EDTA (100, 500, and 1000 g/mL) [27], and AA (10, 50, and 100 g/mL) [28] due to their applications in reducing oxidation and scavenging free radicals in both ophthalmic and non-ophthalmic settings

Site and personnel eligibility The Department of Pediatrics at Kyungpook National University Hospital (KNUH) served as the single study site for this investigation, a tertiary care center with clinical laboratory accreditation and capabilities for pediatric neurodevelopmental assessments, phlebotomy, and secure biobanking

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The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

Zhang H, et al