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After 01-Anisotropy Macro script has finished, navigate to 03-ROI Selection and press Run Using either the oval tool or the any shape tool draw regions of interest over the largest portion of where the sensor is appearing in the Para channel Each independent trial consists of at least five to more than 30 cells (530 technical replicates)

Elevated IGF-1 has theoretical associations with certain proliferative conditions

[1] Cellular Energy Production - Glutathione protects mitochondria (the cell's power plants) from oxidative damage, potentially supporting more efficient energy production during exercise

To minimize oxidation, prepare working solutions immediately before use and avoid prolonged exposure to air

& Moreira, P