Relative expression levels were analyzed using the 2 CT method in Microsoft Excel 2013 (Microsoft Corp., Redmond, USA)
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Once a blood sample is obtained, erythrocyte rGSH is very susceptible to oxidation and the rGSH:GSSH ratio drops rapidly
(7) Samples were then dried using a SpeedVac Plus (Savant, SC110A) and resuspended in 10 L deionized water with 2% (v/v) acetonitrile, 0.05% (v/v) heptafluorobutyric acid (HFBA) and 0.01% (v/v) formic acid