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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

10.1038/jcbfm.2012.126 J

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[50] Patologa [editar] El exceso de glutamato en las sinapsis, que puede liberarse en condiciones tales como en una lesin cerebral traumtica, puede impedir la absorcin de cistena, un componente necesario del glutatin