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Because many PTMs are substoichiometric and transient, enrichment techniques including immunoprecipitation, lectin-based capture, bioorthogonal labeling and metabolic incorporation are commonly employed to isolate modified proteins for MS identification and quantification 5,6,7

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(2016) 150:e1216:12191230.e6

Instead, it constitutes an essential cofactor for the biosynthesis of purine and pyrimidine nucleotides, as well as the amino acids histidine and tryptophan [93]

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