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The resulting buffy coat was collected, resuspended in PBS, and centrifuged again (200 x g, 8 min, 20C, Universal 320, Hettich, Tuttlingen, Germany)

Each time I emailed in I was able to get a response in less than 12 hours

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For patients with genetic variants affecting B12 metabolism or impaired liver function, methylcobalamin is preferable

4 Regulatory mechanisms of L-carnitine on hepatocyte function Clinical evidence indicates that L-carnitine improves liver function in NAFLD patients, reflected by reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (63)