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doi:10.3390/cells13211799

The amino acid glutamate is an essential excitatory neurotransmitter in the human brain that regulates cognitive functions such as learning and memory, which are usually impaired in ASD

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A375 cells were cultured until 3050% confluence, then treated with a complete growth medium containing 0.1 M doxorubicin (Sigma D1515)

Some patients, particularly those with neurological involvement, may benefit from more frequent dosing (every 2 months rather than 3 months), but this should be medically supervised rather than self-adjusted