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2.5 LCMS/MS untargeted metabolomics sequencing 2.5.1 Metabolite extraction Weigh 20 mg of each sample, add 200 L of pre-cooled 80% methanol water with 3 steel beads, homogenize and crush in a tissue crusher, then add 800 L of pre-cooled 80% methanol water, vortex mixing, sonicate for 2 h in an ice bath, and then let stand overnight at 20C