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Everyones hair has a cycle, but the length of the cycle can vary from individual to individual and can be disrupted by a number of factors including age and ill health, to name just a couple of causes

Nails grow faster and may feel stronger, a secondary indicator of systemic collagen metabolism improvement

Dead volume and actual yield Every time you insert a needle into a vial and draw solution, a small amount of liquid remains in the needle hub, in the rubber stopper, and coating the glass walls

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In sensible cosmetic concentrations this is not a general concern, but it argues against casual escalation of dose or concentration in the belief that more is better