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PCR reactions were carried out using 0.5 L of gene-specific cDNA samples and that of 16S rRNA as templates with 1 mM final concentration of each of the forward and reverse RT primers (Additional file 12: Table S5) and 4 L of 2 SYBR green PCR mix in a final volume of 10 L

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[1] For minor concerns such as slight bleeding or bruising at the injection site without systemic symptoms, patients can apply gentle pressure with clean gauze and monitor the area

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Limit high-sugar foods, such as candy, table sugar, syrup, sodas and juices