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Flow cytometry For oral immune cell analysis, mice were sacrificed, and tongue tissue harvested, then mechanically homogenized in RPMI 1640 media and incubated at 37C for 45 min on a GentleMACS Dissociator (Miltenyi Biotec) using the Murine Tissue Dissociation Kit, then passed through a 40 mm cell strainer to form single-cell suspensions
In addition, in prior years we included the final OPPS status indicators and APC assignments in the coding preamble tables, however, because the same information can be found in Addendum B, we no longer include them in Table 12
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If Vitamin B 12 Injection is not clinically indicated you pay only for the consultation
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