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Generating a Doxycycline-resistant MCF7 cell line, to study the potential mechanism(s) underlying drug resistance To study the potential role of Doxycycline-resistance as an escape mechanism during Doxycycline treatment, we created a Doxycycline-resistant MCF7 cell line by serially passaging Doxycycline-sensitive MCF7 cells, in the presence of increasing concentrations of Doxycycline (from 12.5 to 50 M), over a period of 9 weeks

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From there, it factors in your activity level to get your Total Daily Energy Expenditure (TDEE) , then applies a 20% calorie deficit the standard starting point for safe, sustainable fat loss