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Description

Permeabilization assay O/N adjusted C

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ImageJ software (ImageJ software, v1.46r, National Institutes of Health, Bethesda, MD USA) was used to analyze these images and to estimate the area percentage (%) of cleaved caspase-3 and GFAP-positive brown immune-stained cells according to Schneider et al

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Furthermore, cytokines released by astrocytes may bind to their specific receptors in the dopaminergic neurons, such as TNFR1 and 2, and activate proapoptotic mechanisms through the activation of caspase 3, caspase 8, and cytochrome c [10]

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