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The redox potential of roGFP is dependent on the degree of oxidation ( OxD roGFP ) of the redox pair roGFP red /roGFP ox according to Equations 4 and 5: Substitution of Equation 5 into Equation 3 allows transformation of Equation 3 into Equation 6: OxD roGFP can be measured in vitro by monitoring the fluorescence emission intensities ( I ) at 520 nm for excitation at 390 and 480 nm