Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C
Effects of antimicrobial peptides on membrane dynamics: a comparison of fluorescence and NMR experiments
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Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death
10.1007/s00432-009-0562-z [DOI] [PMC free article] [PubMed] [Google Scholar] Deshaware S., Marathe S