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This conformation may also be representative of the peptide agonist conformation of GCGR and other class B GPCRs, although previous models of GLP-1R suggested a more tilted conformation of the ECD relative to the plane of the membrane in the GLP-1-bound state 23,24

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The fold change in expression of each gene was calculated using the comparative DDCt method, with GAPDH as housekeeping reference endogenous control

Plumpton FS, Besser GM