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Following double digestion and purification, both natively paired and combinatorial scFv libraries were generated by ligating 5 g of the appropriate scFv insert into 25 g of the double-digested phagemid vector, LVEC2295 (proprietary to Sanofi), at a phagemid:insert ratio of 1:3 using T4 DNA Ligase (NEB, catalog no

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Circadian Rhythm governs timing of sleep and wake cycles through hormonal cues like melatonin and cortisol

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