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Statistical analysis The planned sample size of 495 participants was estimated to provide over 80% statistical power to detect a difference between any active treatment group and placebo with respect to the primary endpoint using a two-sided t -test with significance level of 0.05

Additionally, adrenaline, which induces cAMP elevation and triggers calcium release from the endoplasmic reticulum, stimulates higher magnitude of calcium oscillation in GCGR-KO cells, indicating increased calcium release from the endoplasmic reticulum

It is also a known strong activator of Nrf2