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Cell viability assays The cells were plated in triplicate in 96-well plates (4000 cells cultured in 2%FBS DMEM and 2000 cells cultured in 10% FBS DMEM)

To determine CI activity (NADH-dependent OCR), MAS plus alamethicin (2.5 g ml 1 ) and cytochrome c (10 g ml 1 ) was added onto the cells followed by 5 mM pyruvate + 5 mM malate + 1 mM NADH + 10 nM recombinant perfringolysin O at port A, 2 M rotenone + 4 M antimycin A at port B, 0.5 mM TMPD + 1 mM ascorbic acid at port C and 50 mM azide at port D

Why lean mass measurements are an imperfect proxy for muscle health and function [21:45]

Up to sevenfold increases were observed at day three