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doi: 10.1042/CS20170134

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doi: 10.1038/s41586-018-0372-z

His-tag is, therefore, often combined with protein tags to enhance soluble expression described above [82,103,104]

In conclusion, the results of the current study allow to speculate that l-ARG might represent a potential alternative for patients with mildmoderate vasculogenic ED with contraindication to PDE5i or who had experienced adverse effects upon PDE5i treatment and might require a different therapeutic approach, or, in association to lower PDE5i doses, to reduce the occurrence and severity of PDE5i-associated adverse effects meanwhile potentially exerting an additive or synergistic effect