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A short procedural review can help teams stay aligned before running repeated administrations: The biggest avoidable error in peptide handling is informal technique

Its biosynthesis is a two-step enzymatic cascade, including first a specific -ligation of glutamate and cysteine by -glutamate-cysteine ligase (GCL) and then the formation of peptide bond between this dipeptide and glycine by glutathione synthetase (GS)

Mutation of the corresponding Arg153 in ScGRX7 confirmed its critical role for the scaffold site as revealed by the 60% reduction of the catalytic efficiency on the substrate Cys-SG 15

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