After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes
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Adipocyte Lipolysis in metabolic signaling Adipocyte Lipolysis in cellular signaling In addition to its metabolic role in providing substrates, lipolysis can also function in signaling to regulate cellular metabolism (Figure 3)