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Insulin-like growth factor 1 (IGF-1) is produced mainly in the liver by growth hormone signaling and is necessary for normal physical growth

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Tissue was enzymically digested by incubation with DM with 10 units/mL papain enzyme (Worthington Biochemicals) for 40 min with regular mixing, followed by two washes with DM and two washes with Neurobasal-A medium (Thermofisher) supplemented with 1% rat serum (Envigo), 1 x B-27 supplement (Thermofisher), 1 x Antimicrobial-Antifungal supplement (premixed penicillin, streptomycin and amphotericin B- Thermofisher) and 1 mM glutamine (Sigma)

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